Abstract:
Background:Local communities in the South Eastern Lowveld of Zimbabwe have adopted the feeding of livestock with Neorautanenia brachypus (Harms) C.A. tuber to mitigate against climate change. Diferences within Neorautane nia brachypus (Harms) tuber fesh colour and preferences by cattle have been observed, suggesting possible diversity within the N. brachypus plant community. This study aimed at distinguishing the N. brachypus wild plant species through phenotypic and genetic characterization using morphological descriptors and random amplifed polymor phic (RAPD) markers respectively. Leaf samples were selected using judgmental sampling techniques from wards 11–15 in Sengwe (Chiredzi district) for leaf morphology and molecular characterization. RAPD-PCR analysis was done using 18-screened random decamer primers to confrm the diversity in the plant population. The similarity of the biotypes was evaluated using binary coding on the basis of the presence or absence of a morphological indicator as well as distinct DNA amplicon fragments. Primer 7.0.13 was used to estimate morphological and genetic similarities using the unweighted pair group method with arithmetic average (UPGMA). The cluster number was estimated using
the Elbow method part of the R package.
Results: Initially, 14 biotype groups were identifed from 96 accessions visually characterized basing of leaf charac teristics. All the leaf biotypes displayed arcuate venation with diferences observed for leaf shape, tip shape and leaf margins. The 14 biotypes clustered into six groups based on the binary data of the morphological characteristics. RAPD primers generated three hundred and sixty eight distinct amplicons with 77.5% being polymorphic from the 14 biotypes. The number of bands produced per primer ranged from four (OPF-02) to 44 (UBC-746). The PIC value ranged from 0.1327 to 0.1873 for the RAPD primers. Use of molecular markers collapsed the biotypes into fve clusters. Both the leaf descriptors and RAPD markers showed the existence of genetic diversity within the wild accessions of N.
brachypus.
Conclusions: A combination of morphological and RAPD markers efectively refned the resolution of the genetic diversity within the N. brachypus wild accessions to nine biotypes. These fndings have indicated to the existence of more than one biotype of N. brachypus with potentially diferent properties. The favorable biotypes can further be promoted through incorporation in pastures as alternative feed or complementary feed to livestock. As such the out put of this study will serve as a guide for N. brachypus germplasm management and improvement.
